Sampling event

HBIs and sterols in zooplankton Nansen Legacy Q3

Latest version published by The Nansen Legacy Project on 11 May 2022 The Nansen Legacy Project
Highly branched isoprenoids (HBIs) and sterols in pelagic zooplankton collected during Nansen Legacy seasonal cruise Q3 (5 - 27 August 2019)
Publication date:
11 May 2022
License:
CC-BY-NC 4.0

Data Records

The data in this sampling event resource has been published as a Darwin Core Archive (DwC-A), which is a standardized format for sharing biodiversity data as a set of one or more data tables. The core data table contains 14 records.

3 extension data tables also exist. An extension record supplies extra information about a core record. The number of records in each extension data table is illustrated below.

  • Event (core)
    14
  • ExtendedMeasurementOrFact 
    1165
  • ResourceRelationship 
    1152
  • Occurrence 
    96

This IPT archives the data and thus serves as the data repository. The data and resource metadata are available for download in the downloads section. The versions table lists other versions of the resource that have been made publicly available and allows tracking changes made to the resource over time.

Downloads

Download the latest version of this resource data as a Darwin Core Archive (DwC-A) or the resource metadata as EML or RTF:

Data as a DwC-A file download 14 records in English (94 kB) - Update frequency: unknown
Metadata as an EML file download in English (17 kB)
Metadata as an RTF file download in English (10 kB)

Versions

The table below shows only published versions of the resource that are publicly accessible.

How to cite

Researchers should cite this work as follows:

Kohlbach D, Wold A, Schmidt K, Smik L, Belt S T, Hop H, Assmy P (2022): HBIs and sterols in zooplankton Nansen Legacy Q3. v1.4. The Nansen Legacy Project. Dataset/Samplingevent. doi: 10.21334/npolar.2022.941be8cc

Rights

Researchers should respect the following rights statement:

The publisher and rights holder of this work is The Nansen Legacy Project. This work is licensed under a Creative Commons Attribution Non Commercial (CC-BY-NC) 4.0 License.

GBIF Registration

This resource has been registered with GBIF, and assigned the following GBIF UUID: 7fcf1da7-2c7a-4e69-a60d-efa5f0b4ec77.  The Nansen Legacy Project publishes this resource, and is itself registered in GBIF as a data publisher endorsed by GBIF Norway.

Keywords

Barents Sea; pelagic zooplankton; highly branched isoprenoids; sterols

Contacts

Who created the resource:

Doreen Kohlbach
Postdoctoral Researcher
Norwegian Polar Institute
Tromsø
NO
Anette Wold
Engineer
Norwegian Polar Institute
TROMSØ
NO
Katrin Schmidt
Researcher
University of Plymouth
Plymouth
GB
Lukas Smik
Postdoctoral Researcher
University of Plymouth
Plymouth
GB
Simon T. Belt
Researcher
University of Plymouth
Plymouth
GB
Haakon Hop
Researcher
Norwegian Polar Institute
Tromsø
NO
Philipp Assmy
Researcher
Norwegian Polar Institute
Tromsø
NO

Who can answer questions about the resource:

Doreen Kohlbach
Postdoctoral Researcher
Norwegian Polar Institute
Tromsø
NO

Who filled in the metadata:

Doreen Kohlbach
Postdoctoral Researcher
Norwegian Polar Institute
Tromsø
NO

Who else was associated with the resource:

Author
Doreen Kohlbach
Postdoctoral Researcher
Norwegian Polar Institute
Tromsø
NO
Author
Anette Wold
Engineer
Norwegian Polar Institute
Tromsø
NO
Author
Katrin Schmidt
Researcher
University of Plymouth
Plymouth
GB
Author
Lukas Smik
Postdoctoral Researcher
University of Plymouth
Plymouth
GB
Author
Simon T. Belt
Researcher
University of Plymouth
Plymouth
GB
Author
Haakon Hop
Researcher
Norwegian Polar Institute
Tromsø
NO
Author
Philipp Assmy
Researcher
Norwegian Polar Institute
Tromsø
NO

Geographic Coverage

Nansen Legacy main transect along 34°E in the Northern Barents Sea and adjacent Arctic Basin from 76 to 82°N

Bounding Coordinates South West [-90, -180], North East [90, 180]

Taxonomic Coverage

Copepods, krill, amphipods, pteropods, cnidarians, ctenophores, chaetognaths, appendicularians

Temporal Coverage

Start Date / End Date 2019-08-05 / 2019-08-27

Sampling Methods

Samples of 23 zooplankton taxa including copepods, krill, amphipods, pteropods and gelatinous species were collected at six stations (P1, P2, P4, P5, P6, P7), using MIK nets (1200 μm with 500 μm cod end) and WP3 net (1000 μm) for large taxa, Macroplankton trawl for mostly Thysanoessa spp. (multiple mesh sizes along the net, tapering to 8 mm at its end), Bongo net (180 μm) and Multinet (180 μm) for copepods (all species), and WP2 net (90 μm) for small copepods. Samples were sorted into the lowest possible taxonomic level, and/or stage/size groups (where possible) onboard the ship and immediately frozen at -80 ºC.

Study Extent Nansen Legacy main transect along 34°E in the Northern Barents Sea and adjacent Arctic Basin from 76 to 82°N

Method step description:

  1. HBIs and sterols were analysed at the University of Plymouth, UK. All samples were freeze-dried (-45 °C, 0.1 mbar, 24-48 h). Total lipids were extracted with chloroform/methanol (2:1, v/v) and cleaned with potassium chloride (0.88 %). Thereafter, samples were saponified with 20 % potassium hydroxide in water/methanol (1:9, v/v) at 70 °C for 1 h. Non-saponifiable lipids were extracted with hexane and purified by open column chromatography filled with SiO2. HBIs were eluted using hexane. The partially purified non-polar lipids containing HBIs were analysed by gas chromatography-mass spectrometry (GC-MS). Quantification of HBIs was achieved by integrating individual ion responses in single-ion monitoring mode, and normalising these to the corresponding peak area of the internal standard and an instrumental response factor obtained from purified standards (Belt et al., 2012). Sterols were eluted from the same silica column using hexane:methylacetate (4:1,v/v). Sterol fractions were derivatised using N,O-bis(trimethylsilyl)trifluoroacetamide (70 °C, 1 h) and analysed GC-MS. Individual sterols were identified by comparison of the mass spectra of their trimethylsilyl-ethers with published data (Belt et al., 2018). Belt, S.T., Brown, T.A., Sanz, P.C., and Rodriguez, A.N. (2012). Structural confirmation of the sea ice biomarker IP25 found in Arctic marine sediments. Environ. Chem. Lett. 10(2), 189-192 Belt, S.T., Brown, T.A., Smik, L., Assmy, P., and Mundy, C.J. (2018). Sterol identification in floating Arctic sea ice algal aggregates and the Antarctic sea ice diatom Berkeleya adeliensis. Org. Geochem. 118, 1-3

Bibliographic Citations

  1. Kohlbach D, Hop H, Wold A, Schmidt K, Smik L, Belt ST, Keck Al-Habahbeh A, Woll M, Graeve M, Dąbrowska AM, Tatarek A, Atkinson A and Assmy P (2021) Multiple Trophic Markers Trace Dietary Carbon Sources in Barents Sea Zooplankton During Late Summer. Front. Mar. Sci. 7:610248. doi: 10.3389/fmars.2020.610248

Additional Metadata