HBIs and sterols in zooplankton Nansen Legacy Q3

Evento de amostragem
Versão mais recente published by The Nansen Legacy Project on mai. 11, 2022 The Nansen Legacy Project
Publication date:
11 de maio de 2022
Licença:
CC-BY-NC 4.0

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Descrição

Highly branched isoprenoids (HBIs) and sterols in pelagic zooplankton collected during Nansen Legacy seasonal cruise Q3 (5 - 27 August 2019)

Registros de Dados

Os dados deste recurso de evento de amostragem foram publicados como um Darwin Core Archive (DwC-A), que é o formato padronizado para compartilhamento de dados de biodiversidade como um conjunto de uma ou mais tabelas de dados. A tabela de dados do núcleo contém 14 registros.

Também existem 3 tabelas de dados de extensão. Um registro de extensão fornece informações adicionais sobre um registro do núcleo. O número de registros em cada tabela de dados de extensão é ilustrado abaixo.

Event (core)
14
ExtendedMeasurementOrFact 
1165
ResourceRelationship 
1152
Occurrence 
96

This IPT archives the data and thus serves as the data repository. The data and resource metadata are available for download in the downloads section. The versions table lists other versions of the resource that have been made publicly available and allows tracking changes made to the resource over time.

Versões

A tabela abaixo mostra apenas versões de recursos que são publicamente acessíveis.

Como citar

Pesquisadores deveriam citar esta obra da seguinte maneira:

Kohlbach D, Wold A, Schmidt K, Smik L, Belt S T, Hop H, Assmy P (2022): HBIs and sterols in zooplankton Nansen Legacy Q3. v1.4. The Nansen Legacy Project. Dataset/Samplingevent. doi: 10.21334/npolar.2022.941be8cc

Direitos

Pesquisadores devem respeitar a seguinte declaração de direitos:

O editor e o detentor dos direitos deste trabalho é The Nansen Legacy Project. This work is licensed under a Creative Commons Attribution Non Commercial (CC-BY-NC 4.0) License.

GBIF Registration

Este recurso foi registrado no GBIF e atribuído ao seguinte GBIF UUID: 7fcf1da7-2c7a-4e69-a60d-efa5f0b4ec77.  The Nansen Legacy Project publica este recurso, e está registrado no GBIF como um publicador de dados aprovado por GBIF Norway.

Palavras-chave

Barents Sea; pelagic zooplankton; highly branched isoprenoids; sterols

Contatos

Doreen Kohlbach
  • Provedor Dos Metadados
  • Autor
  • Originador
  • Ponto De Contato
Postdoctoral Researcher
Norwegian Polar Institute
Tromsø
NO
Anette Wold
  • Autor
  • Originador
Engineer
Norwegian Polar Institute
TROMSØ
NO
Katrin Schmidt
  • Autor
  • Originador
Researcher
University of Plymouth
Plymouth
GB
Lukas Smik
  • Autor
  • Originador
Postdoctoral Researcher
University of Plymouth
Plymouth
GB
Simon T. Belt
  • Autor
Researcher
University of Plymouth
Plymouth
GB
Haakon Hop
  • Autor
Researcher
Norwegian Polar Institute
Tromsø
NO
Philipp Assmy
  • Autor
  • Originador
Researcher
Norwegian Polar Institute
Tromsø
NO
Simon T. Belt
  • Autor
Researcher
University of Plymouth
Plymouth
GB
Haakon Hop
  • Autor
Researcher
Norwegian Polar Institute
Tromsø
NO

Cobertura Geográfica

Nansen Legacy main transect along 34°E in the Northern Barents Sea and adjacent Arctic Basin from 76 to 82°N

Coordenadas delimitadoras Sul Oeste [-90, -180], Norte Leste [90, 180]

Cobertura Taxonômica

Copepods, krill, amphipods, pteropods, cnidarians, ctenophores, chaetognaths, appendicularians

Cobertura Temporal

Data Inicial / Data final 2019-08-05 / 2019-08-27

Métodos de Amostragem

Samples of 23 zooplankton taxa including copepods, krill, amphipods, pteropods and gelatinous species were collected at six stations (P1, P2, P4, P5, P6, P7), using MIK nets (1200 μm with 500 μm cod end) and WP3 net (1000 μm) for large taxa, Macroplankton trawl for mostly Thysanoessa spp. (multiple mesh sizes along the net, tapering to 8 mm at its end), Bongo net (180 μm) and Multinet (180 μm) for copepods (all species), and WP2 net (90 μm) for small copepods. Samples were sorted into the lowest possible taxonomic level, and/or stage/size groups (where possible) onboard the ship and immediately frozen at -80 ºC.

Área de Estudo Nansen Legacy main transect along 34°E in the Northern Barents Sea and adjacent Arctic Basin from 76 to 82°N

Descrição dos passos do método:

  1. HBIs and sterols were analysed at the University of Plymouth, UK. All samples were freeze-dried (-45 °C, 0.1 mbar, 24-48 h). Total lipids were extracted with chloroform/methanol (2:1, v/v) and cleaned with potassium chloride (0.88 %). Thereafter, samples were saponified with 20 % potassium hydroxide in water/methanol (1:9, v/v) at 70 °C for 1 h. Non-saponifiable lipids were extracted with hexane and purified by open column chromatography filled with SiO2. HBIs were eluted using hexane. The partially purified non-polar lipids containing HBIs were analysed by gas chromatography-mass spectrometry (GC-MS). Quantification of HBIs was achieved by integrating individual ion responses in single-ion monitoring mode, and normalising these to the corresponding peak area of the internal standard and an instrumental response factor obtained from purified standards (Belt et al., 2012). Sterols were eluted from the same silica column using hexane:methylacetate (4:1,v/v). Sterol fractions were derivatised using N,O-bis(trimethylsilyl)trifluoroacetamide (70 °C, 1 h) and analysed GC-MS. Individual sterols were identified by comparison of the mass spectra of their trimethylsilyl-ethers with published data (Belt et al., 2018). Belt, S.T., Brown, T.A., Sanz, P.C., and Rodriguez, A.N. (2012). Structural confirmation of the sea ice biomarker IP25 found in Arctic marine sediments. Environ. Chem. Lett. 10(2), 189-192 Belt, S.T., Brown, T.A., Smik, L., Assmy, P., and Mundy, C.J. (2018). Sterol identification in floating Arctic sea ice algal aggregates and the Antarctic sea ice diatom Berkeleya adeliensis. Org. Geochem. 118, 1-3

Citações bibliográficas

  1. Kohlbach D, Hop H, Wold A, Schmidt K, Smik L, Belt ST, Keck Al-Habahbeh A, Woll M, Graeve M, Dąbrowska AM, Tatarek A, Atkinson A and Assmy P (2021) Multiple Trophic Markers Trace Dietary Carbon Sources in Barents Sea Zooplankton During Late Summer. Front. Mar. Sci. 7:610248. doi: 10.3389/fmars.2020.610248

Metadados Adicionais