HBIs and sterols in zooplankton Nansen Legacy Q3

資料紀錄

此資源sampling event的資料已發佈為達爾文核心集檔案（DwC-A），其以一或多組資料表構成分享生物多樣性資料的標準格式。 核心資料表包含 14 筆紀錄。

亦存在 3 筆延伸集的資料表。延伸集中的紀錄補充核心集中紀錄的額外資訊。 每個延伸集資料表中資料筆數顯示如下。

此 IPT 存放資料以提供資料儲存庫服務。資料與資源的詮釋資料可由「下載」單元下載。「版本」表格列出此資源的其它公開版本，以便利追蹤其隨時間的變更。

版本

以下的表格只顯示可公開存取資源的已發布版本。

如何引用

研究者應依照以下指示引用此資源。:

Kohlbach D, Wold A, Schmidt K, Smik L, Belt S T, Hop H, Assmy P (2022): HBIs and sterols in zooplankton Nansen Legacy Q3. v1.4. The Nansen Legacy Project. Dataset/Samplingevent. doi: 10.21334/npolar.2022.941be8cc

權利

研究者應尊重以下權利聲明。:

此資料的發布者及權利單位為 The Nansen Legacy Project。 This work is licensed under a Creative Commons Attribution Non Commercial (CC-BY-NC 4.0) License.

GBIF 註冊

此資源已向GBIF註冊，並指定以下之GBIF UUID: 7fcf1da7-2c7a-4e69-a60d-efa5f0b4ec77。  The Nansen Legacy Project 發佈此資源，並經由GBIF Norway同意向GBIF註冊成為資料發佈者。

關鍵字

Barents Sea; pelagic zooplankton; highly branched isoprenoids; sterols

聯絡資訊

地理涵蓋範圍

Nansen Legacy main transect along 34°E in the Northern Barents Sea and adjacent Arctic Basin from 76 to 82°N

分類群涵蓋範圍

Copepods, krill, amphipods, pteropods, cnidarians, ctenophores, chaetognaths, appendicularians

時間涵蓋範圍

取樣方法

Samples of 23 zooplankton taxa including copepods, krill, amphipods, pteropods and gelatinous species were collected at six stations (P1, P2, P4, P5, P6, P7), using MIK nets (1200 μm with 500 μm cod end) and WP3 net (1000 μm) for large taxa, Macroplankton trawl for mostly Thysanoessa spp. (multiple mesh sizes along the net, tapering to 8 mm at its end), Bongo net (180 μm) and Multinet (180 μm) for copepods (all species), and WP2 net (90 μm) for small copepods. Samples were sorted into the lowest possible taxonomic level, and/or stage/size groups (where possible) onboard the ship and immediately frozen at -80 ºC.

方法步驟描述:

1. HBIs and sterols were analysed at the University of Plymouth, UK. All samples were freeze-dried (-45 °C, 0.1 mbar, 24-48 h). Total lipids were extracted with chloroform/methanol (2:1, v/v) and cleaned with potassium chloride (0.88 %). Thereafter, samples were saponified with 20 % potassium hydroxide in water/methanol (1:9, v/v) at 70 °C for 1 h. Non-saponifiable lipids were extracted with hexane and purified by open column chromatography filled with SiO2. HBIs were eluted using hexane. The partially purified non-polar lipids containing HBIs were analysed by gas chromatography-mass spectrometry (GC-MS). Quantification of HBIs was achieved by integrating individual ion responses in single-ion monitoring mode, and normalising these to the corresponding peak area of the internal standard and an instrumental response factor obtained from purified standards (Belt et al., 2012). Sterols were eluted from the same silica column using hexane:methylacetate (4:1,v/v). Sterol fractions were derivatised using N,O-bis(trimethylsilyl)trifluoroacetamide (70 °C, 1 h) and analysed GC-MS. Individual sterols were identified by comparison of the mass spectra of their trimethylsilyl-ethers with published data (Belt et al., 2018). Belt, S.T., Brown, T.A., Sanz, P.C., and Rodriguez, A.N. (2012). Structural confirmation of the sea ice biomarker IP25 found in Arctic marine sediments. Environ. Chem. Lett. 10(2), 189-192 Belt, S.T., Brown, T.A., Smik, L., Assmy, P., and Mundy, C.J. (2018). Sterol identification in floating Arctic sea ice algal aggregates and the Antarctic sea ice diatom Berkeleya adeliensis. Org. Geochem. 118, 1-3

引用文獻

1. Kohlbach D, Hop H, Wold A, Schmidt K, Smik L, Belt ST, Keck Al-Habahbeh A, Woll M, Graeve M, Dąbrowska AM, Tatarek A, Atkinson A and Assmy P (2021) Multiple Trophic Markers Trace Dietary Carbon Sources in Barents Sea Zooplankton During Late Summer. Front. Mar. Sci. 7:610248. doi: 10.3389/fmars.2020.610248

額外的詮釋資料