Description
HBIs and sterols in pelagic zooplankton collected during Nansen Legacy seasonal cruise Q4 (28 November - 17 December 2019)
Data Records
The data in this sampling event resource has been published as a Darwin Core Archive (DwC-A), which is a standardized format for sharing biodiversity data as a set of one or more data tables. The core data table contains 9 records.
3 extension data tables also exist. An extension record supplies extra information about a core record. The number of records in each extension data table is illustrated below.
This IPT archives the data and thus serves as the data repository. The data and resource metadata are available for download in the downloads section. The versions table lists other versions of the resource that have been made publicly available and allows tracking changes made to the resource over time.
Versions
The table below shows only published versions of the resource that are publicly accessible.
How to cite
Researchers should cite this work as follows:
Kohlbach D, Wold A, Keck Al-Habahbeh A, Schmidt K, Smik L, Belt S T, Hop H, Assmy P (2022): HBIs and sterols in zooplankton Nansen Legacy Q4. v1.2. The Nansen Legacy Project. Dataset/Samplingevent. doi: 10.21334/npolar.2022.37159527
Rights
Researchers should respect the following rights statement:
The publisher and rights holder of this work is The Nansen Legacy Project. This work is licensed under a Creative Commons Attribution Non Commercial (CC-BY-NC 4.0) License.
GBIF Registration
This resource has been registered with GBIF, and assigned the following GBIF UUID: 021366ac-62e5-4acc-a439-4c37b3288d87. The Nansen Legacy Project publishes this resource, and is itself registered in GBIF as a data publisher endorsed by GBIF Norway.
Keywords
Barents Sea; pelagic zooplankton; highly branched isoprenoids; sterols
Contacts
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Geographic Coverage
Nansen Legacy main transect along 34°E in the Northern Barents Sea and adjacent Arctic Basin from 76 to 82°N
| Bounding Coordinates | South West [-90, -180], North East [90, 180] |
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Taxonomic Coverage
Copepods, amphipods
Temporal Coverage
| Start Date / End Date | 2019-11-28 / 2019-12-17 |
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Sampling Methods
Samples of 5 zooplankton taxa including copepods and amphipods were collected at six stations (P1, P4, P5, P6, P7, NLEG3), using MIK nets (1200 μm with 500 μm cod end), Macroplankton trawl (multiple mesh sizes along the net, tapering to 8 mm at its end) and Bongo nets (64 and 180 μm). Samples were sorted into species and stage/size groups onboard the ship and immediately frozen at -80 ºC.
| Study Extent | Nansen Legacy main transect along 34°E in the Northern Barents Sea and adjacent Arctic Basin from 76 to 82°N |
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Method step description:
- HBIs and sterols were analysed at the University of Plymouth, UK. All samples were freeze-dried (-45 °C, 0.1 mbar, 24-48 h). Total lipids were extracted with chloroform/methanol (2:1, v/v) and cleaned with potassium chloride (0.88 %). Thereafter, samples were saponified with 20 % potassium hydroxide in water/methanol (1:9, v/v) at 70 °C for 1 h. Non-saponifiable lipids were extracted with hexane and purified by open column chromatography filled with SiO2. HBIs were eluted using hexane. The partially purified non-polar lipids containing HBIs were analysed by gas chromatography-mass spectrometry (GC-MS). Quantification of HBIs was achieved by integrating individual ion responses in single-ion monitoring mode, and normalising these to the corresponding peak area of the internal standard and an instrumental response factor obtained from purified standards (Belt et al., 2012). Sterols were eluted from the same silica column using hexane:methylacetate (4:1,v/v). Sterol fractions were derivatised using N,O-bis(trimethylsilyl)trifluoroacetamide (70 °C, 1 h) and analysed GC-MS. Individual sterols were identified by comparison of the mass spectra of their trimethylsilyl-ethers with published data (Belt et al., 2018). Belt, S.T., Brown, T.A., Sanz, P.C., and Rodriguez, A.N. (2012). Structural confirmation of the sea ice biomarker IP25 found in Arctic marine sediments. Environ. Chem. Lett. 10(2), 189-192 Belt, S.T., Brown, T.A., Smik, L., Assmy, P., and Mundy, C.J. (2018). Sterol identification in floating Arctic sea ice algal aggregates and the Antarctic sea ice diatom Berkeleya adeliensis. Org. Geochem. 118, 1-3
Bibliographic Citations
- Kohlbach D, Schmidt K, Hop H, Wold A, Al-Habahbeh AK, Belt ST, Woll M, Graeve M, Smik L, Atkinson A and Assmy P (2021) Winter Carnivory and Diapause Counteract the Reliance on Ice Algae by Barents Sea Zooplankton. Front. Mar. Sci. 8:640050 doi: 10.3389/fmars.2021.640050