https://doi.org/10.21334/npolar.2022.37159527
https://ipt.gbif.no/resource?r=hbis_sterols_nansen_legacy_q4
HBIs and sterols in zooplankton Nansen Legacy Q4
Doreen
Kohlbach
Norwegian Polar Institute
Postdoctoral Researcher
Tromsø
NO
doreen.kohlbach@npolar.no
Anette
Wold
Norwegian Polar Institute
Engineer
Tromsø
NO
anette.wold@npolar.no
Amalia
Keck Al-Habahbeh
UiT The Arctic University of Norway
PhD student
Tromsø
NO
amalia.keck@uit.no
Katrin
Schmidt
University of Plymouth
Researcher
Plymouth
GB
katrin.schmidt@plymouth.ac.uk
Lukas
Smik
University of Plymouth
Postdoctoral Researcher
Plymouth
GB
lukas.smik@plymouth.ac.uk
Simon T.
Belt
University of Plymouth
Researcher
Plymouth
GB
s.belt@plymouth.ac.uk
Haakon
Hop
Norwegian Polar Institute
Researcher
Tromsø
NO
haakon.hop@npolar.no
Philipp
Assmy
Norwegian Polar Institute
Researcher
Tromsø
NO
philipp.assmy@npolar.no
Doreen
Kohlbach
Norwegian Polar Institute
Postdoctoral Researcher
Tromsø
NO
doreen.kohlbach@npolar.no
Doreen
Kohlbach
Norwegian Polar Institute
Postdoctoral Researcher
Tromsø
NO
doreen.kohlbach@npolar.no
author
Anette
Wold
Norwegian Polar Institute
Engineer
Tromsø
NO
anette.wold@npolar.no
author
Amalia
Keck Al-Habahbeh
UiT The Arctic University of Norway
PhD student
Tromsø
NO
amalia.keck@uit.no
author
Katrin
Schmidt
University of Plymouth
Researcher
Plymouth
GB
katrin.schmidt@plymouth.ac.uk
author
Lukas
Smik
University of Plymouth
Postdoctoral Researcher
Plymouth
GB
lukas.smik@plymouth.ac.uk
author
Simon T.
Belt
University of Plymouth
Researcher
Plymouth
GB
s.belt@plymouth.ac.uk
author
Haakon
Hop
Norwegian Polar Institute
Researcher
Tromsø
NO
haakon.hop@npolar.no
author
Philipp
Assmy
Norwegian Polar Institute
Researcher
Tromsø
NO
philipp.assmy@npolar.no
author
2022-05-11
eng
HBIs and sterols in pelagic zooplankton collected during Nansen Legacy seasonal cruise Q4 (28 November - 17 December 2019)
Barents Sea
pelagic zooplankton
highly branched isoprenoids
sterols
GBIF Dataset Type Vocabulary: http://rs.gbif.org/vocabulary/gbif/dataset_type_2015-07-10.xml
This work is licensed under a Creative Commons Attribution Non Commercial (CC-BY-NC) 4.0 License.
Nansen Legacy main transect along 34°E in the Northern Barents Sea and adjacent Arctic Basin from 76 to 82°N
-180
180
90
-90
2019-11-28
2019-12-17
Copepods, amphipods
Calanus glacialis
Calanus hyperboreus
Calanus finmarchicus
Themisto abyssorum
Themisto libellula
unkown
Doreen
Kohlbach
Norwegian Polar Institute
Postdoctoral Researcher
Tromsø
NO
doreen.kohlbach@npolar.no
HBIs and sterols were analysed at the University of Plymouth, UK.
All samples were freeze-dried (-45 °C, 0.1 mbar, 24-48 h). Total lipids were extracted with chloroform/methanol (2:1, v/v) and cleaned with potassium chloride (0.88 %). Thereafter, samples were saponified with 20 % potassium hydroxide in water/methanol (1:9, v/v) at 70 °C for 1 h. Non-saponifiable lipids were extracted with hexane and purified by open column chromatography filled with SiO2. HBIs were eluted using hexane. The partially purified non-polar lipids containing HBIs were analysed by gas chromatography-mass spectrometry (GC-MS). Quantification of HBIs was achieved by integrating individual ion responses in single-ion monitoring mode, and normalising these to the corresponding peak area of the internal standard and an instrumental response factor obtained from purified standards (Belt et al., 2012).
Sterols were eluted from the same silica column using hexane:methylacetate (4:1,v/v). Sterol fractions were derivatised using N,O-bis(trimethylsilyl)trifluoroacetamide (70 °C, 1 h) and analysed GC-MS. Individual sterols were identified by comparison of the mass spectra of their trimethylsilyl-ethers with published data (Belt et al., 2018).
Belt, S.T., Brown, T.A., Sanz, P.C., and Rodriguez, A.N. (2012). Structural confirmation of the sea ice biomarker IP25 found in Arctic marine sediments. Environ. Chem. Lett. 10(2), 189-192
Belt, S.T., Brown, T.A., Smik, L., Assmy, P., and Mundy, C.J. (2018). Sterol identification in floating Arctic sea ice algal aggregates and the Antarctic sea ice diatom Berkeleya adeliensis. Org. Geochem. 118, 1-3
Nansen Legacy main transect along 34°E in the Northern Barents Sea and adjacent Arctic Basin from 76 to 82°N
Samples of 5 zooplankton taxa including copepods and amphipods were collected at six stations (P1, P4, P5, P6, P7, NLEG3), using MIK nets (1200 μm with 500 μm cod end), Macroplankton trawl (multiple mesh sizes along the net, tapering to 8 mm at its end) and Bongo nets (64 and 180 μm). Samples were sorted into species and stage/size groups onboard the ship and immediately frozen at -80 ºC.
2022-04-29T07:48:35.992+00:00
dataset
Kohlbach D, Wold A, Keck Al-Habahbeh A, Schmidt K, Smik L, Belt S T, Hop H, Assmy P (2022): HBIs and sterols in zooplankton Nansen Legacy Q4. v1.2. The Nansen Legacy Project. Dataset/Samplingevent. doi: 10.21334/npolar.2022.37159527
Kohlbach D, Schmidt K, Hop H, Wold A, Al-Habahbeh AK, Belt ST, Woll M, Graeve M, Smik L, Atkinson A and Assmy P (2021) Winter Carnivory and Diapause Counteract the Reliance on Ice Algae by Barents Sea Zooplankton. Front. Mar. Sci. 8:640050
https://ipt.gbif.no/resource?id=hbis_sterols_nansen_legacy_q4/v1.2.xml