https://doi.org/10.21334/npolar.2022.37159527 https://ipt.gbif.no/resource?r=hbis_sterols_nansen_legacy_q4 HBIs and sterols in zooplankton Nansen Legacy Q4 Doreen Kohlbach Norwegian Polar Institute Postdoctoral Researcher
Tromsø NO
doreen.kohlbach@npolar.no
Anette Wold Norwegian Polar Institute Engineer
Tromsø NO
anette.wold@npolar.no
Amalia Keck Al-Habahbeh UiT The Arctic University of Norway PhD student
Tromsø NO
amalia.keck@uit.no
Katrin Schmidt University of Plymouth Researcher
Plymouth GB
katrin.schmidt@plymouth.ac.uk
Lukas Smik University of Plymouth Postdoctoral Researcher
Plymouth GB
lukas.smik@plymouth.ac.uk
Simon T. Belt University of Plymouth Researcher
Plymouth GB
s.belt@plymouth.ac.uk
Haakon Hop Norwegian Polar Institute Researcher
Tromsø NO
haakon.hop@npolar.no
Philipp Assmy Norwegian Polar Institute Researcher
Tromsø NO
philipp.assmy@npolar.no
Doreen Kohlbach Norwegian Polar Institute Postdoctoral Researcher
Tromsø NO
doreen.kohlbach@npolar.no
Doreen Kohlbach Norwegian Polar Institute Postdoctoral Researcher
Tromsø NO
doreen.kohlbach@npolar.no author
Anette Wold Norwegian Polar Institute Engineer
Tromsø NO
anette.wold@npolar.no author
Amalia Keck Al-Habahbeh UiT The Arctic University of Norway PhD student
Tromsø NO
amalia.keck@uit.no author
Katrin Schmidt University of Plymouth Researcher
Plymouth GB
katrin.schmidt@plymouth.ac.uk author
Lukas Smik University of Plymouth Postdoctoral Researcher
Plymouth GB
lukas.smik@plymouth.ac.uk author
Simon T. Belt University of Plymouth Researcher
Plymouth GB
s.belt@plymouth.ac.uk author
Haakon Hop Norwegian Polar Institute Researcher
Tromsø NO
haakon.hop@npolar.no author
Philipp Assmy Norwegian Polar Institute Researcher
Tromsø NO
philipp.assmy@npolar.no author
2022-05-11 eng HBIs and sterols in pelagic zooplankton collected during Nansen Legacy seasonal cruise Q4 (28 November - 17 December 2019) Barents Sea pelagic zooplankton highly branched isoprenoids sterols GBIF Dataset Type Vocabulary: http://rs.gbif.org/vocabulary/gbif/dataset_type_2015-07-10.xml This work is licensed under a Creative Commons Attribution Non Commercial (CC-BY-NC) 4.0 License. Nansen Legacy main transect along 34°E in the Northern Barents Sea and adjacent Arctic Basin from 76 to 82°N -180 180 90 -90 2019-11-28 2019-12-17 Copepods, amphipods Calanus glacialis Calanus hyperboreus Calanus finmarchicus Themisto abyssorum Themisto libellula unkown Doreen Kohlbach Norwegian Polar Institute Postdoctoral Researcher
Tromsø NO
doreen.kohlbach@npolar.no
HBIs and sterols were analysed at the University of Plymouth, UK. All samples were freeze-dried (-45 °C, 0.1 mbar, 24-48 h). Total lipids were extracted with chloroform/methanol (2:1, v/v) and cleaned with potassium chloride (0.88 %). Thereafter, samples were saponified with 20 % potassium hydroxide in water/methanol (1:9, v/v) at 70 °C for 1 h. Non-saponifiable lipids were extracted with hexane and purified by open column chromatography filled with SiO2. HBIs were eluted using hexane. The partially purified non-polar lipids containing HBIs were analysed by gas chromatography-mass spectrometry (GC-MS). Quantification of HBIs was achieved by integrating individual ion responses in single-ion monitoring mode, and normalising these to the corresponding peak area of the internal standard and an instrumental response factor obtained from purified standards (Belt et al., 2012). Sterols were eluted from the same silica column using hexane:methylacetate (4:1,v/v). Sterol fractions were derivatised using N,O-bis(trimethylsilyl)trifluoroacetamide (70 °C, 1 h) and analysed GC-MS. Individual sterols were identified by comparison of the mass spectra of their trimethylsilyl-ethers with published data (Belt et al., 2018). Belt, S.T., Brown, T.A., Sanz, P.C., and Rodriguez, A.N. (2012). Structural confirmation of the sea ice biomarker IP25 found in Arctic marine sediments. Environ. Chem. Lett. 10(2), 189-192 Belt, S.T., Brown, T.A., Smik, L., Assmy, P., and Mundy, C.J. (2018). Sterol identification in floating Arctic sea ice algal aggregates and the Antarctic sea ice diatom Berkeleya adeliensis. Org. Geochem. 118, 1-3 Nansen Legacy main transect along 34°E in the Northern Barents Sea and adjacent Arctic Basin from 76 to 82°N Samples of 5 zooplankton taxa including copepods and amphipods were collected at six stations (P1, P4, P5, P6, P7, NLEG3), using MIK nets (1200 μm with 500 μm cod end), Macroplankton trawl (multiple mesh sizes along the net, tapering to 8 mm at its end) and Bongo nets (64 and 180 μm). Samples were sorted into species and stage/size groups onboard the ship and immediately frozen at -80 ºC.
2022-04-29T07:48:35.992+00:00 dataset Kohlbach D, Wold A, Keck Al-Habahbeh A, Schmidt K, Smik L, Belt S T, Hop H, Assmy P (2022): HBIs and sterols in zooplankton Nansen Legacy Q4. v1.2. The Nansen Legacy Project. Dataset/Samplingevent. doi: 10.21334/npolar.2022.37159527 Kohlbach D, Schmidt K, Hop H, Wold A, Al-Habahbeh AK, Belt ST, Woll M, Graeve M, Smik L, Atkinson A and Assmy P (2021) Winter Carnivory and Diapause Counteract the Reliance on Ice Algae by Barents Sea Zooplankton. Front. Mar. Sci. 8:640050 https://ipt.gbif.no/resource?id=hbis_sterols_nansen_legacy_q4/v1.2.xml