Fatty acids in zooplankton Nansen Legacy Q3

Sampling event
Latest version published by The Nansen Legacy Project on May 11, 2022 The Nansen Legacy Project
Publication date:
11 May 2022
CC-BY-NC 4.0

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Relative proportions of fatty acids in zooplankton collected during Nansen Legacy seasonal cruise Q3 (5 - 27 August 2019)

Data Records

The data in this sampling event resource has been published as a Darwin Core Archive (DwC-A), which is a standardized format for sharing biodiversity data as a set of one or more data tables. The core data table contains 14 records.

2 extension data tables also exist. An extension record supplies extra information about a core record. The number of records in each extension data table is illustrated below.

Event (core)

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How to cite

Researchers should cite this work as follows:

Kohlbach D, Wold A, Graeve M, Hop H, Assmy P (2022): Fatty acids in zooplankton Nansen Legacy Q3. v1.6. The Nansen Legacy Project. Dataset/Samplingevent. doi: 10.21334/npolar.2022.53bfa233


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The publisher and rights holder of this work is The Nansen Legacy Project. This work is licensed under a Creative Commons Attribution Non Commercial (CC-BY-NC 4.0) License.

GBIF Registration

This resource has been registered with GBIF, and assigned the following GBIF UUID: 34ed3819-a996-47ad-bc36-ee0a8471a57d.  The Nansen Legacy Project publishes this resource, and is itself registered in GBIF as a data publisher endorsed by GBIF Norway.


Barents Sea; pelagic zooplankton; fatty acids


Doreen Kohlbach
  • Metadata Provider
  • Author
  • Originator
  • Point Of Contact
Postdoctoral Researcher
Norwegian Polar Institute
Anette Wold
  • Author
  • Originator
Norwegian Polar Institute
Martin Graeve
  • Author
  • Originator
Alfred Wegener Institute
Haakon Hop
  • Author
  • Originator
Norwegian Polar Institute
Philipp Assmy
  • Author
  • Originator
Norwegian Polar Institute

Geographic Coverage

Nansen Legacy main transect along 34°E in the Northern Barents Sea and adjacent Arctic Basin from 76 to 82°N

Bounding Coordinates South West [-90, -180], North East [90, 180]

Taxonomic Coverage

Copepods, krill, amphipods, pteropods, cnidarians, ctenophores, chaetognaths, appendicularians

Temporal Coverage

Start Date / End Date 2019-08-05 / 2019-08-27

Sampling Methods

Samples of 24 zooplankton taxa including copepods, krill, amphipods, pteropods and gelatinous species were collected at six stations (P1, P2, P4, P5, P6, P7), using MIK nets (1200 μm with 500 μm cod end) and WP3 net (1000 μm) for large taxa, Macroplankton trawl for mostly Thysanoessa spp. (multiple mesh sizes along the net, tapering to 8 mm at its end), Bongo net (180 μm) and Multinet (180 μm) for copepods (all species), and WP2 net (90 μm) for small copepods. Samples were sorted into the lowest possible taxonomic level, and/or stage/size groups (where possible) onboard the ship and immediately frozen at -80 ºC.

Study Extent Nansen Legacy main transect along 34°E in the Northern Barents Sea and adjacent Arctic Basin from 76 to 82°N

Method step description:

  1. Fatty acids were analysed at the Alfred Wegener Institute, Bremerhaven, Germany. All samples were freeze-dried for at least 24 h prior to lipid extraction (-45 °C, 0.1 mbar). Zooplankton were homogenized mechanically using a Potter-Elvehjem homogenizer. Total lipids were extracted using a modified procedure from Folch et al. (1957) with dichloromethane/methanol (2:1, v/v). The extracted lipids were cleaned with 0.88 % potassium chloride solution and converted into fatty acid methyl esters (FAMEs) and free fatty alcohols derived from wax esters by transesterification in methanol, containing 3 % concentrated sulfuric acid, at 80 °C for 4 h. After a subsequent hexane extraction, the FAMEs and alcohols were separated split-less via gas chromatography. FAMEs were identified via standard mixtures and total lipid content was quantified with an internal standard (23:0) that was added prior to lipid extraction. Folch, J., Lees, M., and Stanley, G.H.S. (1957). A simple method for the isolation and purification of total lipides from animal tissues. J. Biol. Chem. 226(1), 497-509

Bibliographic Citations

  1. Kohlbach D, Hop H, Wold A, Schmidt K, Smik L, Belt ST, Keck Al-Habahbeh A, Woll M, Graeve M, Dąbrowska AM, Tatarek A, Atkinson A and Assmy P (2021) Multiple Trophic Markers Trace Dietary Carbon Sources in Barents Sea Zooplankton During Late Summer. Front. Mar. Sci. 7:610248. doi: 10.3389/fmars.2020.610248

Additional Metadata

Alternative Identifiers https://doi.org/10.21334/npolar.2022.53bfa233