Fatty acids in zooplankton Nansen Legacy Q3

Registros de Dados

Os dados deste recurso de evento de amostragem foram publicados como um Darwin Core Archive (DwC-A), que é o formato padronizado para compartilhamento de dados de biodiversidade como um conjunto de uma ou mais tabelas de dados. A tabela de dados do núcleo contém 14 registros.

Também existem 2 tabelas de dados de extensão. Um registro de extensão fornece informações adicionais sobre um registro do núcleo. O número de registros em cada tabela de dados de extensão é ilustrado abaixo.

This IPT archives the data and thus serves as the data repository. The data and resource metadata are available for download in the downloads section. The versions table lists other versions of the resource that have been made publicly available and allows tracking changes made to the resource over time.

Versões

A tabela abaixo mostra apenas versões de recursos que são publicamente acessíveis.

Como citar

Pesquisadores deveriam citar esta obra da seguinte maneira:

Kohlbach D, Wold A, Graeve M, Hop H, Assmy P (2022): Fatty acids in zooplankton Nansen Legacy Q3. v1.6. The Nansen Legacy Project. Dataset/Samplingevent. doi: 10.21334/npolar.2022.53bfa233

Direitos

Pesquisadores devem respeitar a seguinte declaração de direitos:

O editor e o detentor dos direitos deste trabalho é The Nansen Legacy Project. This work is licensed under a Creative Commons Attribution Non Commercial (CC-BY-NC 4.0) License.

GBIF Registration

Este recurso foi registrado no GBIF e atribuído ao seguinte GBIF UUID: 34ed3819-a996-47ad-bc36-ee0a8471a57d.  The Nansen Legacy Project publica este recurso, e está registrado no GBIF como um publicador de dados aprovado por GBIF Norway.

Palavras-chave

Barents Sea; pelagic zooplankton; fatty acids

Contatos

Cobertura Geográfica

Nansen Legacy main transect along 34°E in the Northern Barents Sea and adjacent Arctic Basin from 76 to 82°N

Cobertura Taxonômica

Copepods, krill, amphipods, pteropods, cnidarians, ctenophores, chaetognaths, appendicularians

Cobertura Temporal

Métodos de Amostragem

Samples of 24 zooplankton taxa including copepods, krill, amphipods, pteropods and gelatinous species were collected at six stations (P1, P2, P4, P5, P6, P7), using MIK nets (1200 μm with 500 μm cod end) and WP3 net (1000 μm) for large taxa, Macroplankton trawl for mostly Thysanoessa spp. (multiple mesh sizes along the net, tapering to 8 mm at its end), Bongo net (180 μm) and Multinet (180 μm) for copepods (all species), and WP2 net (90 μm) for small copepods. Samples were sorted into the lowest possible taxonomic level, and/or stage/size groups (where possible) onboard the ship and immediately frozen at -80 ºC.

Descrição dos passos do método:

1. Fatty acids were analysed at the Alfred Wegener Institute, Bremerhaven, Germany. All samples were freeze-dried for at least 24 h prior to lipid extraction (-45 °C, 0.1 mbar). Zooplankton were homogenized mechanically using a Potter-Elvehjem homogenizer. Total lipids were extracted using a modified procedure from Folch et al. (1957) with dichloromethane/methanol (2:1, v/v). The extracted lipids were cleaned with 0.88 % potassium chloride solution and converted into fatty acid methyl esters (FAMEs) and free fatty alcohols derived from wax esters by transesterification in methanol, containing 3 % concentrated sulfuric acid, at 80 °C for 4 h. After a subsequent hexane extraction, the FAMEs and alcohols were separated split-less via gas chromatography. FAMEs were identified via standard mixtures and total lipid content was quantified with an internal standard (23:0) that was added prior to lipid extraction. Folch, J., Lees, M., and Stanley, G.H.S. (1957). A simple method for the isolation and purification of total lipides from animal tissues. J. Biol. Chem. 226(1), 497-509

Citações bibliográficas

1. Kohlbach D, Hop H, Wold A, Schmidt K, Smik L, Belt ST, Keck Al-Habahbeh A, Woll M, Graeve M, Dąbrowska AM, Tatarek A, Atkinson A and Assmy P (2021) Multiple Trophic Markers Trace Dietary Carbon Sources in Barents Sea Zooplankton During Late Summer. Front. Mar. Sci. 7:610248. doi: 10.3389/fmars.2020.610248

Metadados Adicionais