說明
Relative proportions of fatty acids in zooplankton collected during Nansen Legacy seasonal cruise Q3 (5 - 27 August 2019)
資料紀錄
此資源sampling event的資料已發佈為達爾文核心集檔案（DwC-A），其以一或多組資料表構成分享生物多樣性資料的標準格式。 核心資料表包含 14 筆紀錄。
亦存在 2 筆延伸集的資料表。延伸集中的紀錄補充核心集中紀錄的額外資訊。 每個延伸集資料表中資料筆數顯示如下。
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版本
以下的表格只顯示可公開存取資源的已發布版本。
如何引用
研究者應依照以下指示引用此資源。:
Kohlbach D, Wold A, Graeve M, Hop H, Assmy P (2022): Fatty acids in zooplankton Nansen Legacy Q3. v1.6. The Nansen Legacy Project. Dataset/Samplingevent. doi: 10.21334/npolar.2022.53bfa233
權利
研究者應尊重以下權利聲明。:
此資料的發布者及權利單位為 The Nansen Legacy Project。 This work is licensed under a Creative Commons Attribution Non Commercial (CC-BY-NC 4.0) License.
GBIF 註冊
此資源已向GBIF註冊，並指定以下之GBIF UUID: 34ed3819-a996-47ad-bc36-ee0a8471a57d。 The Nansen Legacy Project 發佈此資源，並經由GBIF Norway同意向GBIF註冊成為資料發佈者。
關鍵字
Barents Sea; pelagic zooplankton; fatty acids
聯絡資訊
- 元數據提供者 ●
- 作者 ●
- 出處 ●
- 連絡人
地理涵蓋範圍
Nansen Legacy main transect along 34°E in the Northern Barents Sea and adjacent Arctic Basin from 76 to 82°N
| 界定座標範圍 | 緯度南界 經度西界 [-90, -180], 緯度北界 經度東界 [90, 180] |
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分類群涵蓋範圍
Copepods, krill, amphipods, pteropods, cnidarians, ctenophores, chaetognaths, appendicularians
時間涵蓋範圍
| 起始日期 / 結束日期 | 2019-08-05 / 2019-08-27 |
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取樣方法
Samples of 24 zooplankton taxa including copepods, krill, amphipods, pteropods and gelatinous species were collected at six stations (P1, P2, P4, P5, P6, P7), using MIK nets (1200 μm with 500 μm cod end) and WP3 net (1000 μm) for large taxa, Macroplankton trawl for mostly Thysanoessa spp. (multiple mesh sizes along the net, tapering to 8 mm at its end), Bongo net (180 μm) and Multinet (180 μm) for copepods (all species), and WP2 net (90 μm) for small copepods. Samples were sorted into the lowest possible taxonomic level, and/or stage/size groups (where possible) onboard the ship and immediately frozen at -80 ºC.
| 研究範圍 | Nansen Legacy main transect along 34°E in the Northern Barents Sea and adjacent Arctic Basin from 76 to 82°N |
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方法步驟描述:
- Fatty acids were analysed at the Alfred Wegener Institute, Bremerhaven, Germany. All samples were freeze-dried for at least 24 h prior to lipid extraction (-45 °C, 0.1 mbar). Zooplankton were homogenized mechanically using a Potter-Elvehjem homogenizer. Total lipids were extracted using a modified procedure from Folch et al. (1957) with dichloromethane/methanol (2:1, v/v). The extracted lipids were cleaned with 0.88 % potassium chloride solution and converted into fatty acid methyl esters (FAMEs) and free fatty alcohols derived from wax esters by transesterification in methanol, containing 3 % concentrated sulfuric acid, at 80 °C for 4 h. After a subsequent hexane extraction, the FAMEs and alcohols were separated split-less via gas chromatography. FAMEs were identified via standard mixtures and total lipid content was quantified with an internal standard (23:0) that was added prior to lipid extraction. Folch, J., Lees, M., and Stanley, G.H.S. (1957). A simple method for the isolation and purification of total lipides from animal tissues. J. Biol. Chem. 226(1), 497-509
引用文獻
- Kohlbach D, Hop H, Wold A, Schmidt K, Smik L, Belt ST, Keck Al-Habahbeh A, Woll M, Graeve M, Dąbrowska AM, Tatarek A, Atkinson A and Assmy P (2021) Multiple Trophic Markers Trace Dietary Carbon Sources in Barents Sea Zooplankton During Late Summer. Front. Mar. Sci. 7:610248. doi: 10.3389/fmars.2020.610248
額外的詮釋資料
| 替代的識別碼 | https://doi.org/10.21334/npolar.2022.53bfa233 |
|---|---|
| 34ed3819-a996-47ad-bc36-ee0a8471a57d | |
| https://ipt.gbif.no/resource?r=fatty_acids_nl_q3 |