說明
Relative proportions of lipid classes in pelagic zooplankton collected during Nansen Legacy seasonal cruise Q4 (28 November - 17 December 2019)
資料紀錄
此資源sampling event的資料已發佈為達爾文核心集檔案（DwC-A），其以一或多組資料表構成分享生物多樣性資料的標準格式。 核心資料表包含 8 筆紀錄。
亦存在 2 筆延伸集的資料表。延伸集中的紀錄補充核心集中紀錄的額外資訊。 每個延伸集資料表中資料筆數顯示如下。
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版本
以下的表格只顯示可公開存取資源的已發布版本。
如何引用
研究者應依照以下指示引用此資源。:
Kohlbach D, Wold A, Keck Al-Habahbeh A, Woll M, Graeve M, Hop H, Assmy P (2022): Lipid classes in zooplankton Nansen Legacy Q4. v1.2. The Nansen Legacy Project. Dataset/Samplingevent. doi: 10.21334/npolar.2022.d436b683
權利
研究者應尊重以下權利聲明。:
此資料的發布者及權利單位為 The Nansen Legacy Project。 This work is licensed under a Creative Commons Attribution Non Commercial (CC-BY-NC 4.0) License.
GBIF 註冊
此資源已向GBIF註冊，並指定以下之GBIF UUID: 95e464b4-e286-414b-9c59-6fdc9a4afc14。 The Nansen Legacy Project 發佈此資源，並經由GBIF Norway同意向GBIF註冊成為資料發佈者。
關鍵字
Barents Sea; pelagic zooplankton; lipid classes; neutral lipids; polar lipids
聯絡資訊
- 元數據提供者 ●
- 作者 ●
- 出處 ●
- 連絡人
地理涵蓋範圍
Nansen Legacy main transect along 34°E in the Northern Barents Sea and adjacent Arctic Basin from 76 to 82°N
| 界定座標範圍 | 緯度南界 經度西界 [-90, -180], 緯度北界 經度東界 [90, 180] |
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分類群涵蓋範圍
Copepods, amphipods
時間涵蓋範圍
| 起始日期 / 結束日期 | 2019-11-28 / 2019-12-17 |
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取樣方法
Samples of 5 zooplankton taxa including copepods and amphipods were collected at six stations (P1, P4, P5, P6, P7, NLEG3), using MIK nets (1200 μm with 500 μm cod end), Macroplankton trawl (multiple mesh sizes along the net, tapering to 8 mm at its end) and Bongo nets (64 and 180 μm). Samples were sorted into species and stage/size groups onboard the ship and immediately frozen at -80 ºC.
| 研究範圍 | Nansen Legacy main transect along 34°E in the Northern Barents Sea and adjacent Arctic Basin from 76 to 82°N |
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方法步驟描述:
- Lipid classes were analysed at the Alfred Wegener Institute, Bremerhaven, Germany. All samples were freeze-dried for at least 24 h prior to lipid extraction (-45 °C, 0.1 mbar). Zooplankton were homogenized mechanically using a Potter-Elvehjem homogenizer. Total lipids were extracted using a modified procedure from Folch et al. (1957) with dichloromethane/methanol (2:1, v/v). The extracted lipids were cleaned with 0.88 % potassium chloride solution. Lipid class analysis was performed directly on the extracted lipids (Graeve and Janssen, 2009) via high performance liquid chromatography. Lipid classes were distinguished into neutral (i.e. storage) lipids and polar (i.e. membrane) lipids. Folch, J., Lees, M., and Stanley, G.H.S. (1957). A simple method for the isolation and purification of total lipides from animal tissues. J. Biol. Chem. 226(1), 497-509 Graeve, M., and Janssen, D. (2009). Improved separation and quantification of neutral and polar lipid classes by HPLC–ELSD using a monolithic silica phase: application to exceptional marine lipids. J. Chromatogr. B 877(20-21), 1815-1819
引用文獻
- Kohlbach D, Schmidt K, Hop H, Wold A, Al-Habahbeh AK, Belt ST, Woll M, Graeve M, Smik L, Atkinson A and Assmy P (2021) Winter Carnivory and Diapause Counteract the Reliance on Ice Algae by Barents Sea Zooplankton. Front. Mar. Sci. 8:640050 doi: 10.3389/fmars.2021.640050