The data in this sampling event resource has been published as a Darwin Core Archive (DwC-A), which is a standardized format for sharing biodiversity data as a set of one or more data tables. The core data table contains 8 records.
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How to cite
Researchers should cite this work as follows:
Kohlbach D, Wold A, Keck Al-Habahbeh A, Woll M, Graeve M, Hop H, Assmy P (2022): Lipid classes in zooplankton Nansen Legacy Q4. v1.2. The Nansen Legacy Project. Dataset/Samplingevent. doi: 10.21334/npolar.2022.d436b683
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The publisher and rights holder of this work is The Nansen Legacy Project. This work is licensed under a Creative Commons Attribution Non Commercial (CC-BY-NC) 4.0 License.
This resource has been registered with GBIF, and assigned the following GBIF UUID: 95e464b4-e286-414b-9c59-6fdc9a4afc14. The Nansen Legacy Project publishes this resource, and is itself registered in GBIF as a data publisher endorsed by GBIF Norway.
Barents Sea; pelagic zooplankton; lipid classes; neutral lipids; polar lipids
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Nansen Legacy main transect along 34°E in the Northern Barents Sea and adjacent Arctic Basin from 76 to 82°N
|Bounding Coordinates||South West [-90, -180], North East [90, 180]|
|Start Date / End Date||2019-11-28 / 2019-12-17|
Samples of 5 zooplankton taxa including copepods and amphipods were collected at six stations (P1, P4, P5, P6, P7, NLEG3), using MIK nets (1200 μm with 500 μm cod end), Macroplankton trawl (multiple mesh sizes along the net, tapering to 8 mm at its end) and Bongo nets (64 and 180 μm). Samples were sorted into species and stage/size groups onboard the ship and immediately frozen at -80 ºC.
|Study Extent||Nansen Legacy main transect along 34°E in the Northern Barents Sea and adjacent Arctic Basin from 76 to 82°N|
Method step description:
- Lipid classes were analysed at the Alfred Wegener Institute, Bremerhaven, Germany. All samples were freeze-dried for at least 24 h prior to lipid extraction (-45 °C, 0.1 mbar). Zooplankton were homogenized mechanically using a Potter-Elvehjem homogenizer. Total lipids were extracted using a modified procedure from Folch et al. (1957) with dichloromethane/methanol (2:1, v/v). The extracted lipids were cleaned with 0.88 % potassium chloride solution. Lipid class analysis was performed directly on the extracted lipids (Graeve and Janssen, 2009) via high performance liquid chromatography. Lipid classes were distinguished into neutral (i.e. storage) lipids and polar (i.e. membrane) lipids. Folch, J., Lees, M., and Stanley, G.H.S. (1957). A simple method for the isolation and purification of total lipides from animal tissues. J. Biol. Chem. 226(1), 497-509 Graeve, M., and Janssen, D. (2009). Improved separation and quantification of neutral and polar lipid classes by HPLC–ELSD using a monolithic silica phase: application to exceptional marine lipids. J. Chromatogr. B 877(20-21), 1815-1819
- Kohlbach D, Schmidt K, Hop H, Wold A, Al-Habahbeh AK, Belt ST, Woll M, Graeve M, Smik L, Atkinson A and Assmy P (2021) Winter Carnivory and Diapause Counteract the Reliance on Ice Algae by Barents Sea Zooplankton. Front. Mar. Sci. 8:640050 doi: 10.3389/fmars.2021.640050