https://doi.org/10.21334/npolar.2022.40c7af2a
https://ipt.gbif.no/resource?r=fatty_acids_nansen_legacy_q4
Fatty acids in zooplankton Nansen Legacy Q4
Doreen
Kohlbach
Norwegian Polar Institute
Postdoctoral Researcher
Tromsø
NO
doreen.kohlbach@npolar.no
Anette
Wold
Norwegian Polar Institute
Engineer
Tromsø
NO
anette.wold@npolar.no
Amalia
Keck Al-Habahbeh
UiT The Arctic University of Norway
PhD student
Tromsø
NO
amalia.keck@uit.no
Martin
Graeve
Alfred Wegener Institute
Researcher
Bremerhaven
DE
martin.graeve@awi.de
Haakon
Hop
Norwegian Polar Institute
Researcher
Tromsø
NO
haakon.hop@npolar.no
Philipp
Assmy
Norwegian Polar Institute
Researcher
Tromsø
NO
philipp.assmy@npolar.no
Doreen
Kohlbach
Norwegian Polar Institute
Postdoctoral Researcher
Tromsø
NO
doreen.kohlbach@npolar.no
Doreen
Kohlbach
Norwegian Polar Institute
Postdoctoral Researcher
Tromsø
NO
doreen.kohlbach@npolar.no
author
Anette
Wold
Norwegian Polar Institute
Engineer
Tromsø
NO
anette.wold@npolar.no
author
Amalia
Keck Al-Habahbeh
UiT The Arctic University of Norway
PhD student
Tromsø
NO
amalia.keck@uit.no
author
Martin
Graeve
Alfred Wegener Institute
Researcher
Bremerhaven
DE
martin.graeve@awi.de
author
Haakon
Hop
Norwegian Polar Institute
Researcher
Tromsø
NO
haakon.hop@npolar.no
author
Philipp
Assmy
Norwegian Polar Institute
Researcher
Tromsø
NO
philipp.assmy@npolar.no
author
2022-05-11
eng
Relative proportions of fatty acids in pelagic zooplankton collected during Nansen Legacy cruise Q4 (28 November to 17 December)
Barents Sea
pelagic zooplankton
fatty acids
GBIF Dataset Type Vocabulary: http://rs.gbif.org/vocabulary/gbif/dataset_type_2015-07-10.xml
This work is licensed under a Creative Commons Attribution Non Commercial (CC-BY-NC) 4.0 License.
Nansen Legacy main transect along 34°E in the Northern Barents Sea and adjacent Arctic Basin from 76 to 82°N
-180
180
90
-90
2019-11-28
2019-12-17
Copepods, amphipods
Calanus glacialis
Calanus hyperboreus
Calanus finmarchicus
Themisto abyssorum
Themisto libellula
unkown
Doreen
Kohlbach
Norwegian Polar Institute
Postdoctoral Researcher
Tromsø
NO
doreen.kohlbach@npolar.no
Fatty acids were analysed at the Alfred Wegener Institute, Bremerhaven, Germany.
All samples were freeze-dried for at least 24 h prior to lipid extraction (-45 °C, 0.1 mbar). Zooplankton were homogenized mechanically using a Potter-Elvehjem homogenizer. Total lipids were extracted using a modified procedure from Folch et al. (1957) with dichloromethane/methanol (2:1, v/v). The extracted lipids were cleaned with 0.88 % potassium chloride solution and converted into fatty acid methyl esters (FAMEs) and free fatty alcohols derived from wax esters by transesterification in methanol, containing 3 % concentrated sulfuric acid, at 80 °C for 4 h. After a subsequent hexane extraction, the FAMEs and alcohols were separated split-less via gas chromatography. FAMEs were identified via standard mixtures and total lipid content was quantified with an internal standard (23:0) that was added prior to lipid extraction.
Folch, J., Lees, M., and Stanley, G.H.S. (1957). A simple method for the isolation and purification of total lipides from animal tissues. J. Biol. Chem. 226(1), 497-509
Nansen Legacy main transect along 34°E in the Northern Barents Sea and adjacent Arctic Basin from 76 to 82°N
Samples of 5 zooplankton taxa including copepods and amphipods were collected at six stations (P1, P4, P5, P6, P7, NLEG3), using MIK nets (1200 μm with 500 μm cod end), Macroplankton trawl (multiple mesh sizes along the net, tapering to 8 mm at its end) and Bongo nets (64 and 180 μm). Samples were sorted into species and stage/size groups onboard the ship and immediately frozen at -80 ºC.
2022-04-28T09:38:46.947+00:00
dataset
Kohlbach D, Wold A, Keck Al-Habahbeh A, Graeve M, Hop H, Assmy P (2022): Fatty acids in zooplankton Nansen Legacy Q4. v1.3. The Nansen Legacy Project. Dataset/Samplingevent. doi: 10.21334/npolar.2022.40c7af2a
Kohlbach D, Schmidt K, Hop H, Wold A, Al-Habahbeh AK, Belt ST, Woll M, Graeve M, Smik L, Atkinson A and Assmy P (2021) Winter Carnivory and Diapause Counteract the Reliance on Ice Algae by Barents Sea Zooplankton. Front. Mar. Sci. 8:640050
https://ipt.gbif.no/resource?id=fatty_acids_nansen_legacy_q4/v1.3.xml