https://doi.org/10.21334/npolar.2022.40c7af2a https://ipt.gbif.no/resource?r=fatty_acids_nansen_legacy_q4 Fatty acids in zooplankton Nansen Legacy Q4 Doreen Kohlbach Norwegian Polar Institute Postdoctoral Researcher
Tromsø NO
doreen.kohlbach@npolar.no
Anette Wold Norwegian Polar Institute Engineer
Tromsø NO
anette.wold@npolar.no
Amalia Keck Al-Habahbeh UiT The Arctic University of Norway PhD student
Tromsø NO
amalia.keck@uit.no
Martin Graeve Alfred Wegener Institute Researcher
Bremerhaven DE
martin.graeve@awi.de
Haakon Hop Norwegian Polar Institute Researcher
Tromsø NO
haakon.hop@npolar.no
Philipp Assmy Norwegian Polar Institute Researcher
Tromsø NO
philipp.assmy@npolar.no
Doreen Kohlbach Norwegian Polar Institute Postdoctoral Researcher
Tromsø NO
doreen.kohlbach@npolar.no
Doreen Kohlbach Norwegian Polar Institute Postdoctoral Researcher
Tromsø NO
doreen.kohlbach@npolar.no author
Anette Wold Norwegian Polar Institute Engineer
Tromsø NO
anette.wold@npolar.no author
Amalia Keck Al-Habahbeh UiT The Arctic University of Norway PhD student
Tromsø NO
amalia.keck@uit.no author
Martin Graeve Alfred Wegener Institute Researcher
Bremerhaven DE
martin.graeve@awi.de author
Haakon Hop Norwegian Polar Institute Researcher
Tromsø NO
haakon.hop@npolar.no author
Philipp Assmy Norwegian Polar Institute Researcher
Tromsø NO
philipp.assmy@npolar.no author
2022-05-11 eng Relative proportions of fatty acids in pelagic zooplankton collected during Nansen Legacy cruise Q4 (28 November to 17 December) Barents Sea pelagic zooplankton fatty acids GBIF Dataset Type Vocabulary: http://rs.gbif.org/vocabulary/gbif/dataset_type_2015-07-10.xml This work is licensed under a Creative Commons Attribution Non Commercial (CC-BY-NC) 4.0 License. Nansen Legacy main transect along 34°E in the Northern Barents Sea and adjacent Arctic Basin from 76 to 82°N -180 180 90 -90 2019-11-28 2019-12-17 Copepods, amphipods Calanus glacialis Calanus hyperboreus Calanus finmarchicus Themisto abyssorum Themisto libellula unkown Doreen Kohlbach Norwegian Polar Institute Postdoctoral Researcher
Tromsø NO
doreen.kohlbach@npolar.no
Fatty acids were analysed at the Alfred Wegener Institute, Bremerhaven, Germany. All samples were freeze-dried for at least 24 h prior to lipid extraction (-45 °C, 0.1 mbar). Zooplankton were homogenized mechanically using a Potter-Elvehjem homogenizer. Total lipids were extracted using a modified procedure from Folch et al. (1957) with dichloromethane/methanol (2:1, v/v). The extracted lipids were cleaned with 0.88 % potassium chloride solution and converted into fatty acid methyl esters (FAMEs) and free fatty alcohols derived from wax esters by transesterification in methanol, containing 3 % concentrated sulfuric acid, at 80 °C for 4 h. After a subsequent hexane extraction, the FAMEs and alcohols were separated split-less via gas chromatography. FAMEs were identified via standard mixtures and total lipid content was quantified with an internal standard (23:0) that was added prior to lipid extraction. Folch, J., Lees, M., and Stanley, G.H.S. (1957). A simple method for the isolation and purification of total lipides from animal tissues. J. Biol. Chem. 226(1), 497-509 Nansen Legacy main transect along 34°E in the Northern Barents Sea and adjacent Arctic Basin from 76 to 82°N Samples of 5 zooplankton taxa including copepods and amphipods were collected at six stations (P1, P4, P5, P6, P7, NLEG3), using MIK nets (1200 μm with 500 μm cod end), Macroplankton trawl (multiple mesh sizes along the net, tapering to 8 mm at its end) and Bongo nets (64 and 180 μm). Samples were sorted into species and stage/size groups onboard the ship and immediately frozen at -80 ºC.
2022-04-28T09:38:46.947+00:00 dataset Kohlbach D, Wold A, Keck Al-Habahbeh A, Graeve M, Hop H, Assmy P (2022): Fatty acids in zooplankton Nansen Legacy Q4. v1.3. The Nansen Legacy Project. Dataset/Samplingevent. doi: 10.21334/npolar.2022.40c7af2a Kohlbach D, Schmidt K, Hop H, Wold A, Al-Habahbeh AK, Belt ST, Woll M, Graeve M, Smik L, Atkinson A and Assmy P (2021) Winter Carnivory and Diapause Counteract the Reliance on Ice Algae by Barents Sea Zooplankton. Front. Mar. Sci. 8:640050 https://ipt.gbif.no/resource?id=fatty_acids_nansen_legacy_q4/v1.3.xml